Sampling for qPCR Analysis

It is important that samples taken for qPCR analysis are collected and shipped properly because the data obtained can be compromised by inadequate collection and shipment. MI can analyze almost any type of sample matrix for qPCR including water, soil/sediment, biofilms, enrichment cultures and more. Following are links to pdf files which provide detailed descriptions on how to collect groundwater, soil/sediment, and bio-trap samplers for qPCR.

Bio-Flo Sampling Protocol for Q-Potential (DNA)
Detailed instructions on how to use the bio-flo sampling approach to collect groundwater samples using a variety pumps (peristaltic preferred, grundfos, air bladder, etc) for Q-Potential (DNA).

Bio-Flo Sampling Protocol for Q-Expression (RNA)
Detailed instructions on how to use the bio-flo sampling approach to collect groundwater samples using a variety pumps (peristaltic preferred, grundfos, air bladder, etc) for Q-Expression (RNA).

Bio-Trap Sampler Protocol for Q-Potential (DNA)
Detailed instructions on how to use the Bio-Trap Samplers for Q-Potential (DNA).

Bio-Trap Sampler Protocol for Q-Expression (RNA)
Detailed instructions on how to use the Bio-Trap Samplers for Q-Expression (RNA).

Soil/Sediment Sampling Protocol for Q-Potential (DNA)
Detailed instructions on how to collect soil/sediment samples for DGGE Analysis.

Other Useful Forms

MI Chain of Custody
A chain of custody (COC) from must be included with each shipment of samples. Please package the COC in a moisture-proof bag and attach on the underside of the cooler lid. Click on the pdf link to obtain a copy of our COC or contact us to have a copy faxed to you.

Soil Import Permit Label
For convenient shipping of international samples Microbial Insights Inc. holds a soil import permit (#S-4574).