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Why is the 16S rRNA gene used for identification?

Date added:
Thursday, 05 February 2009

Answer

The 16S rRNA gene is found in all bacteria.  It has been widely accepted as an excellent marker for identfying bacteria due to the following reasons:

  • 16S rRNA genes are found in all prokaryotes (bacteria and archaea)
  • 16S rRNA gene sequences differ between species
  • Some sections of the 16S rRNA gene sequence are highly conserved (similiar) whereas others differ greatly.
  • 16S rRNA gene sequences have been used to produce extensive bacterial databases which facilitate our ability to rapidly identify specific organisms.

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