Denaturing
gradient gel electrophoresis (DGGE) is a nucleic acid based (DNA or
RNA) technique which can be used to profile and identify dominant
members of the microbial community based on their genetic fingerprint.| Click for details on how the analysis
is performed
DGGE profiles can be produced for a variety of
target groups including:
For more information please click on
each target group.
Using this approach,
differences in the genetic sequence of the 16S rRNA gene
(28S for fungi) allow DNA from various microorganisms to physically
separate and generate a profile of the dominant members which become
visible as bands in a gel image. The banding patterns and relative
intensities of the bands provide a measure of difference among the
communities. Bands from microorganisms which constitute at
least 1-2% of the total community are excised and sequence analysis is
used to determine the identity of that microorganism.
Applications
include:
Determine Indentity of
dominant species within a microbial community
Evaluate similarities/differences within
species composition between different samples/treatments
