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How is SIP performed?

Date added:
Thursday, 05 February 2009

Answer

SIP is performed using Bio-Trap samplers that have been "baited" with a known concentration of a 13C enriched contaminant (i.e. benzene, MTBE, etc.). These samplers are placed in selected monitoring wells and allowed to incubate for ~30 days in order to allow microbial growth. Incubation times can vary depending upon scope of the study. Following incubation, the Bio-Trap samplers are removed from the well and shipped to Microbial Insights for analysis.

Bio-Traps are a unique sampling system which offers many advantages for microbial analysis. The Bio-Sep beads within the Bio-Trap provide a large surface area for colonization of actively growing microbes, they also allow for easy application of amendments (in this case with the 13C-labeled compound of interest).

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