How is SIP performed?
- Date added:
- Thursday, 05 February 2009
Answer
SIP is performed using Bio-Trap samplers that have been "baited" with a known concentration of a 13C enriched contaminant (i.e. benzene, MTBE, etc.). These samplers are placed in selected monitoring wells and allowed to incubate for ~30 days in order to allow microbial growth. Incubation times can vary depending upon scope of the study. Following incubation, the Bio-Trap samplers are removed from the well and shipped to Microbial Insights for analysis.
Bio-Traps are a unique sampling system which offers many advantages for microbial analysis. The Bio-Sep beads within the Bio-Trap provide a large surface area for colonization of actively growing microbes, they also allow for easy application of amendments (in this case with the 13C-labeled compound of interest).



