How does NGS-ER work?

While each NGS platform is unique, the overall steps and the underlying concepts are similar.  DNA is extracted from the sample and fragmented into a library of small segments that are amplified and subsequently sequenced in millions of parallel reactions.  The sequencing step is similar to previous methods – the bases of each DNA fragment are sequentially identified from light signals emitted as the complement to each fragment strand is re-synthesized.  The net result is a set of newly identified strings of nucleotides called “reads” that represent members of the microbial community present in the original sample.