Association between Dehalococcoides spp. Concentrations and Other Site Parameters

Greg Davis, Brett Baldwin and Dora Ogles (Microbial Insights, Inc.)

Substantial research has been conducted to understand the microbiology of reductive dechlorination. This research has lead to the isolation of genera known to dechlorinate (e.g. Dehalococcoides, Dehalobacter, etc.) as well as the isolation of specific functional genes (tceA,bvcA, vcrA) associated with certain steps in the dechlorination process. Recently, modern molecular techniques such as Real-Time PCR (CENSUS®) have been applied to quantify the abundance of specific bacterial groups involved in reductive dechlorination and also to quantify functional genes of interest.

Understanding the association between known dechlorinating bacteria such as Dehalococcoides spp. and other site parameters (contaminant concentration, geochemistry, etc.) could provide essential information for effective site management and decision making. Results on the distribution of known dechlorinating bacteria in association with
other site specific parameters have been combined into a database. This presentation will discuss the trends that have been observed thus far, based on data from over 1,000 samples from a wide range of sites.

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Abstract B-115, in: Bruce M. Sass (Conference Chair), Remediation of Chlorinated and Recalcitrant Compound—2008.
Proceedings of the Sixth International Conference on Remediation of Chlorinated and Recalcitrant Compounds (Monterey, CA; May 2008). ISBN 1-57477-163-9, published by Battelle, Columbus, OH, www.battelle.org/chlorcon.