QuantArray-MIC

Simultaneously quantify microbes involved in Microbiologically Influenced Corrosion and Oilfield Souring in a single analysis.

Microbial influenced corrosion (MIC) impacts nearly all industries and can exact a severe toll in terms of loss of production, O&M costs, deterioration of equipment and potentially the health, safety, and environmental consequences of corrosion related failure.  MIC and souring are complex processes that depend on the actions and interactions of diverse bacterial communities of not only sulfate reducing bacteria but also acid producing bacteria, methanogens, iron oxidizers, slime formers, denitrifiers, and sulfur oxidizing bacteria.  The QuantArray provides simultaneous quantification of key organisms and functional genes involved in MIC as well as oilfield souring proving a more comprehensive assessment.  All of the organisms listed below are included in a single QuantArray analysis.

Evaluation of the microorganisms present within a system permits managers to:

  • Quantify MIC bacteria and accurately assess corrosion potential
  • Evaluate the effectiveness of treatments and O&M measures
Target   Relevance / Data Interpretation
Total Eubacteria  MIC is initiated by growth of a biofilm on the material surface. Monitoring total bacteria provides a general measure for evaluating bacterial growth in the system.
Total Archaea Archaea are another general group of single celled microorganisms which, like bacteria, can initiate and contribute to MIC. Depending upon types and environmental conditions, total archaea can outnumber total bacteria and be a more important factor in MIC.
Sulfate Reducing Bacteria Sulfate reducing bacteria (SRB) consume hydrogen, produce hydrogen sulfide and are the group of microorganism most commonly implicated in the pitting corrosion of various metals.
Sulfate Reducing Archaea Sulfate reducing archaea consume hydrogen, produce hydrogen sulfide and have been implicated in MIC at elevated temperatures.
 Exopolysaccaride Production Gene involved in the production of exopolysaccharide (EPS) and biofilm formation by some Burkholderia spp.
Methanogens Methanogens utilize hydrogen for growth, can contribute to cathodic depolarization and can cause corrosion rates comparable to sulfate reducing bacteria.
Fermenting Bacteria
Anaerobic bacteria produce organic acids and hydrogen.  Acid production can lead to localized drops in pH facilitating corrosion while hydrogen production can support growth of other MIC associated organisms including SRB.
Nitrate Reducing Bacteria Increasingly, nitrate addition is being used to stimulate growth of nitrate reducing bacteria as a bioexclusion strategy to combat SRB-mediated reservoir souring and MIC. The qDNF assay quantifies target genes encoding enzymes responsible for a key step in biological nitrate reduction.
Acid Producing Bacteria Acetogenic bacteria are strict anaerobes that produce acetate from the conversion of H2-CO2, CO, or formate. Hydrogen mediated acetogenesis has been demonstrated in high pressure natural gas pipelines confirming the in situ activity of this bacterial group. Further, the presence of acetic acid is known to exacerbate carbon dioxide corrosion of carbon steel.
Iron Oxidizing Bacteria Iron oxidizing bacteria are a group of microorganisms commonly implicated in metal deposition and tubercle formation.
Manganese Oxidizing Bacteria Like iron oxidizing bacteria, manganese oxidizing bacteria are capable of making deposits of metal oxides.
Sulfur Oxidizing Bacteria Often aerobic bacteria oxidize sulfide or elemental sulfur producing sulfuric acid.  Commonly implicated in the corrosion of concrete.
Iron Reducing Bacteria (other)
Iron reducing bacteria reduce insoluble ferric iron to soluble ferrous iron potentially facilitating the removal of protective corrosion products formed on exposed iron alloy surfaces.  However, other studies have suggested that the actions of IRB can inhibit corrosion through a variety of mechanisms.  This assay targets iron reducing bacteria such as Deferribacter, Ferrimonas, Geopsychrobacter, Geothermobacter, Geothrix, Geovibrio, Geothermobacterium and Albidiferax.  Please note that Geobacter and Shewanella  are also common iron reducing bacteria which need to be ordered as separate assays.
Iron Reducing Bacteria (Geobacter) Iron reducing bacteria reduce insoluble ferric iron to soluble ferrous iron potentially facilitating the removal of protective corrosion products formed on exposed iron alloy surfaces.  This assay targets a common iron reducing bacteria, Geobacter. 
Iron Reducing Bacteria (Shewanella) Anaerobic bacteria which can utilize cathodic hydrogen as an energy source, reduce ferric iron and sulfite to ferrous iron and sulfide indicating that it can play a role in MIC.
Iron Reducing Archaea Targets two genera of iron reducing archaea, Ferroglobus and Geoglobus.
Nitrogen Fixing Bacteria Nitrogen fixation converts nitrogen gas into ammonia which can be assimilated by organisms.  Nitrogen fixation may become increasingly important in mature biofilms.
Ammonia Oxidizing Bacteria Ammonia oxidation or nitrification produces nitric acid causing corrosion of concrete and natural stone.  Depending on alkalinity levels, nitrification in water systems can increase lead contamination and increase copper solubility.
Deinococcus spp.
Genus of bacteria considered very efficient primary biofilm formers and therefore have been implicated in slime formation and biofouling.
Meiothermus spp. Like Deinococcus spp., Meiothermus spp. are efficient primary biofilm formers and frequently implicated in slime formation and biofouling.