|An increasing number of microorganisms have been isolated that utilize dioxane as a growth supporting substrate under aerobic conditions suggesting biodegradation is a viable attenuation mechanism. In the aerobic dioxane utilizing microorganism Pseudonocardia dioxanivorans CB1190, the first step in dioxane metabolism is mediated by a dioxane/tetrahydrofuran monooxygenase (DXMO/THFMO). An aldehyde dehydrogenase (ALDH) is co-expressed with DXMO/THFMO.|
Therefore, qPCR assays have been developed to quantify the DXMO/THFMO and ALDH genes to evaluate the potential for dioxane/tetrahydrofuran (co)metabolism in environmental samples (Gedalanga et al. 2014).