Dehalogenimonas spp. couple growth to dichloroelimination of a variety of vicinally chlorinated ethanes including 1,2-DCA, 1,1,2-TCA, 1,1,2,2-TeCA and chlorinated propanes such as 1,2-DCP and 1,2,3-TCP (Dillehay et al. 2014; Moe et al. 2009).
Therefore, Microbial Insights (MI) recommends CENSUS® qPCR or QuantArray® quantification of Dehalogenimonas spp. to evaluate the potential for anaerobic biodegradation at sites impacted by chlorinated ethanes or chlorinated propanes.
In addition to Dehalogenimonas, MI recommends quantification of Dehalococcoides, vinyl chloride reductase genes, and Dehalobacter spp. at chlorinated ethane sites. As with Dehalogenimonas, Dehalobacter spp. can utilize chlorinated ethanes as growth supporting electron acceptors. Quantification of Dehalococcoides and functional genes is recommended at chlorinated ethane sites because biodegradation of 1,1,2,2-tetrachloroethane and 1,1,2-trichloroethane by Dehalogenimonas and Dehalobacter spp. proceeds by dichloroelimination producing dichloroethene (cis- and trans-DCE) and vinyl chloride as shown below (Moe et al. 2009; Yan et al. 2009).