How does CENSUS work?
|CENSUS is based on a revolutionary molecular biological tool (MBT) called quantitative polymerase chain reaction (qPCR) whereby many copies of a specific gene are generated. As each gene copy is made, a fluorescent marker is released, measured, and used to quantify the number of target genes present in the sample. The gene copied during the process (target) is determined by short segments of DNA called “primers” which are added to the reaction mixture. In essence, qPCR is like a copy machine with a counter. The “primers” select which pages (target) of the book (DNA) are copied and the counter keeps a running total of how many pages were copied (number of target genes in the sample).|