Next-Generation DNA Sequencing (NGS)
Profile and Identify Dominant Members of a Microbial Community
Next-generation DNA sequencing (NGS) is a collective term for a group of advanced technologies that allow the determination of the exact order of nucleotides present in a DNA molecule. No other technique yet developed offers the ability to better examine microbial community composition in environmental samples and answer the question: Who is there?
Identification: NGS also commonly called metagenomics provides identification of microorganisms present in an environmental sample often down to the genus level with no prior knowledge of the microbial community composition. Although function cannot always be predicted from phylogeny, comprehensive identification of the microorganisms present offers unprecedented insight into the potential microbial processes occurring in the environment.
Profile Comparisons: Comparisons of next-generation sequencing results between samples can reveal important differences or shifts in the microbial community by location, over time, or in response to site activities.